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Sperm egg binding

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We have attempted to identify a surface component of echinoderm eggs that is involved in the species-specific binding of sperm. Cell surface membranes from eggs of the sea urchins Strongylocentrotus purpuratus or Arbacia punctulata were radioiodinated, detergent-treated, and subjected to density-gradient centrifugation. In the presence of bindin, the complementary binding protein isolated from sperm, one component of the membranes sedimented to a different density. This membrane component bound species specifically to sperm that had undergone the acrosome reaction.
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Avian Reproduction

Metrics details. Genes that encode proteins associated with sperm competition, fertilization, and sexual conflicts of interest are often among the most rapidly evolving parts of animal genomes. One family of sperm-expressed genes Zp3r , C4bpa in the mammalian gene cluster called the regulator of complement activation RCA encodes proteins that bind eggs and mediate reproductive success, and are therefore expected to show high relative rates of nonsynonymous nucleotide substitution in response to sexual selection in comparison to other genes not involved in gamete binding at fertilization. We tested that working hypothesis by using phylogenetic models of codon evolution to identify episodes of diversifying positive selection. We used a comparative approach to quantify the evidence for episodic diversifying selection acting on RCA genes with known functions in fertilization and sensitivity to sexual selection , and contrast them with other RCA genes in the same gene family that function in innate immunity and are not sensitive to sexual selection.
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Animal Reproduction Science

Fertilization i. Several components on the sperm surface play an active role in mediating the binding and fusion of the gametes. Specific domains on the sperm membrane equatorial segment and posterior head and the oocyte membrane microvillar region are involved in this process Evans et al. The purpose of this paper is to examine in greater detail the sperm molecules involved in sperm-egg binding, in particular the fertilin molecule originally termed PH
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That is the main difference between them. Even though the goal of both fertilization methods is the same, i. However, in practice, people tend to use the abbreviation IVF to refer to the former, whilst ICSI is employed to make reference to the latter.

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